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Protein Separations Using Colloidal Magnetic Nanoparticles
Author(s) -
Bucak Seyda,
Jones Deverraux A.,
Laibinis Paul E.,
Hatton T. Alan
Publication year - 2003
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0200853
Subject(s) - adsorption , colloid , nanoparticle , magnetite , chromatography , magnetic nanoparticles , chemistry , filtration (mathematics) , size exclusion chromatography , chemical engineering , ion exchange , colloidal particle , magnetite nanoparticles , phospholipid , magnetic separation , materials science , ion , membrane , nanotechnology , organic chemistry , biochemistry , enzyme , statistics , mathematics , engineering , metallurgy
Phospholipid‐coated colloidal magnetic nanoparticles with mean magnetite core size of 8 nm are shown to be effective ion exchange media for the recovery and separation of proteins from protein mixtures. These particles have high adsorptive capacities (up to 1200 mg protein/mL adsorbent, an order of magnitude larger than the best commercially available adsorbents) and exhibit none of the diffusional resistances offered by conventional porous ion exchange media. Protein‐laden particles are readily recovered from the feed solution using high‐gradient magnetic filtration.