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Continuous Production of High‐Content Fructooligosaccharides by a Complex Cell System
Author(s) -
Sheu DeyChyi,
Duan KowJen,
Cheng ChihYu,
Bi JiaLin,
Chen JerYi
Publication year - 2002
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp020081y
Subject(s) - aureobasidium pullulans , chemistry , gluconic acid , bioreactor , sucrose , food science , chromatography , calcium , continuous production , biochemistry , fermentation , organic chemistry , environmental engineering , engineering
A complex biocatalyst system with a bioreactor equipped with a microfiltration (MF) module was employed to produce high‐content fructooligosaccharides (FOS) in a continuous process initiated by a batch process. The system used mycelia of Aspergillus japonicus CCRC 93007 or Aureobasidium pullulans ATCC 9348 with β‐fructofuranosidase activity and Gluconobacter oxydans ATCC 23771 with glucose dehydrogenase activity. Calcium carbonate slurry was used to control pH to 5.5, and gluconic acid in the reaction mixture was precipitated as calcium gluconate. Sucrose solution with an optimum concentration of 30% (w/v) was employed as feed for the complex cell system, and high‐content FOS was discharged continuously from a MF module. The complex cell system was run at 30 °C with an aeration rate of 5 vvm and produced more than 80% FOS with the remainder being 5–7% glucose and 8–10% sucrose on a dry weight basis, plus a small amount of calcium gluconate. The system worked for a 7‐day continuous production process with a dilution rate of 0.04 h −1 , and the volumetric productivity for total FOS was more than 160 g L −1 h −1 .