Premium
Highly Multilayered Urease Decomposes Highly Concentrated Urea
Author(s) -
Kobayashi Satoshi,
Yonezu Shinji,
Kawakita Hidetaka,
Saito Kyoichi,
Sugita Kazuyuki,
Tamada Masao,
Sugo Takanobu,
Lee William
Publication year - 2003
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp020072p
Subject(s) - urease , urea , chemistry , hydrolysis , permeation , membrane , ion exchange , chromatography , polymer , nuclear chemistry , inorganic chemistry , ion , biochemistry , organic chemistry
Urease was immobilized at a density of 1.2 g of urease per gram of a matrix via ion‐exchange binding of urease to an anion‐exchange polymer chain grafted onto a pore surface of a porous hollow‐fiber membrane and subsequent cross‐linking of urease with transglutaminase. Urea was hydrolyzed during the permeation of a urea solution, the concentration of which ranged from 2 to 8 M, through the pores of the resultant membrane with a thickness of approximately 1 mm. Quantitative hydrolysis of 4 M urea was achieved at a permeation rate lower than 1 mL/h, i.e., a residence time longer than 5.1 min, at ambient temperature. This performance is ascribed to convective transport of urea through the pores rimmed by the urease‐immobilized polymer chains at a high density. Urease was denatured in the presence of urea at concentrations higher than 6 M while hydrolyzing urea.