Premium
Optimization of High Molecular Weight Pullulan Production by Aureobasidium pullulans in Batch Fermentations
Author(s) -
Gibson Larry H.,
Coughlin Robert W.
Publication year - 2002
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0200043
Subject(s) - aureobasidium pullulans , pullulan , titer , chemistry , food science , molar mass , fermentation , chromatography , biology , polysaccharide , biochemistry , organic chemistry , antibody , immunology , polymer
Of five strains of Aureobasidium pullulans studied, NRRL Y‐2311–1 yielded the highest titer (26.2 g/L) of pullulan and formed the lowest amount of melanin‐like pigment. Sucrose was superior to glucose as the carbon and energy source on the basis of yield and titer of pullulan produced. Pullulan titer was higher (26.2 vs 5.1 g/L), biomass concentration was lower (6.9 vs 12.7 g/L), and DO was lower (0 vs 60% of saturation) when the fermenter was agitated by a marine propeller compared to Rushton impellers. Pullulan produced by strain NRRL Y‐2311–1 ranged in weight‐average molar mass ( M w ) from 486 KDa and number‐average molar mass ( M n ) from 220 Da on day 1 of growth to 390 KDa and 690 Da on day 6; M w declined by about 35% from day 1 to day 3, the day of maximum pullulan titer. For the other strains, the ranges of molar mass on the day of maximum pullulan titer were 338–614 KDa ( M w ) and 100–6820 Da ( M n ).