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Penicillin G Acylase‐Fatty Lipid Biocomposite Films Show Excellent Catalytic Activity and Long Term Stability/Reusability
Author(s) -
Phadtare Sumant,
Parekh Parag,
Gole Anand,
Patil Mrunal,
Pundle Archana,
Prabhune Asmita,
Sastry Murali
Publication year - 2002
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp015504v
Subject(s) - biocomposite , immobilized enzyme , chemistry , enzyme assay , thermal stability , fourier transform infrared spectroscopy , chemical engineering , lipase , enzyme , biocatalysis , chromatography , biochemistry , organic chemistry , materials science , catalysis , ionic liquid , composite number , engineering , composite material
The formation of biocomposite films of the pharmaceutically important enzyme penicillin G acylase (PGA) and fatty lipids under enzyme‐friendly conditions is described. The approach involves a simple beaker‐based diffusion protocol wherein the enzyme diffuses into the lipid film during immersion in the enzyme solution, thereby leading to the formation of a biocomposite film. The incorporation of the enzyme in both cationic as well as anionic lipids suggests the important role of secondary interactions such as hydrophobic and hydrogen bonding in the enzyme immobilization process. The kinetics of formation of the enzyme‐lipid biocomposites has been studied by quartz crystal microgravimentry (QCM) measurements. The stability of the enzyme in the lipid matrix was confirmed by Fourier transform infrared spectroscopy (FTIR) and biocatalytic activity measurements. Whereas the biological activity of the lipid‐immobilized enzyme was marginally higher than that of the free enzyme, the biocomposite film exhibited increased thermal/temporal stability. Particularly exciting was the observation that the biocomposite films could be reused in biocatalysis reactions without significant loss in activity, which indicates potentially exciting biomedical/industrial application of these films.