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Use of At‐Line Spectrophotometry for the Rapid Definition of Pilot‐Scale Flocculation Processes
Author(s) -
BouHabib G.,
Yeung K.,
TitchenerHooker N. J.,
Hoare M.
Publication year - 2002
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp010185t
Subject(s) - flocculation , process engineering , process (computing) , contamination , scale (ratio) , biological system , downstream processing , process control , environmental science , computer science , chemistry , biochemical engineering , chromatography , engineering , environmental engineering , physics , ecology , quantum mechanics , biology , operating system
Traditionally most downstream bioprocesses have been operated without real‐time knowledge of product and key contaminants, yielding little confidence in their operation and the impact on subsequent operations. A rapid UV–vis spectral prediction technique has been successfully demonstrated for the at‐line characterization of a large scale continuous flocculation process in terms of RNA, key protein contaminants, and cell debris. A comparison was made between the spectral predictions and retrospective wet chemical assays, and a highly linear correlation was obtained. The spectral analysis technique allowed for real‐time system information, which was applied to control the flocculation process to maintain satisfactory process performance, even when subjected to given possible process disturbances.