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Influence of Fungal Elicitors on Production of Ajmalicine by Cell Cultures of Catharanthus roseus
Author(s) -
Namdeo Ajay,
Patil Shridhar,
Fulzele Devanand P.
Publication year - 2002
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0101280
Subject(s) - catharanthus roseus , elicitor , trichoderma viride , aspergillus niger , biology , fusarium , alkaloid , microbiology and biotechnology , botany , food science , biochemistry , enzyme
Suspension cultures of Catharanthus roseus ( C. roseus ) were elicited with fungal cell wall fragments of Aspergillus niger (A.niger ), Fusarium moniliforme (F. moniliforme ), and Trichoderma viride (T.viride ). The effects of elicitor dosage, exposures time, and age of subculture on ajmalicine accumulation were studied. A higher concentration of elicitor extract responded positively to C. roseus suspension cultures. Ajmalicine accumulation increased by about 3‐fold when cells were treated with A.niger , F. moniliforme , and T. viride. The maximum ajmalicine production (75 μg g −1 dry weight (DW)) was observed in cells treated with T. viride . Cell cultures were elicited with 5% preparation of A. niger , F. moniliforme , and T. viride and exposed for 24, 48, 72, and 96 h. for elicitation. Suspension cultures elicited with T. viride for 48 h showed a 3‐fold increase (87 μg g −1 DW) in ajmalicine contents, whereas A. niger and F. moniliforme synthesized a 2‐fold increase in alkaloid and yielded 52 and 56 μg g −1 DW ajmalicine, respectively. C. roseus cells of different age (5,10, 15, 20, and 25 days old) were treated with a 5% elicitor of A. niger , F. moniliforme , and T. viride and investigated elicitors activity at different age of cell cultures. Maximum yield 166 μg g −1 DW of ajmalicine was synthesized in 20 day old suspension cultures treated with T. viride. A longer period of incubation of cell cultures with elicitors adversely affected the ajmalicine synthesis.