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Analysis of Biofilm Structure and Gene Expression Using Fluorescence Dual Labeling
Author(s) -
Gilbert Eric S.,
Khlebnikov Artem,
Cowan Stacie E.,
Keasling Jay D.
Publication year - 2001
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0101031
Subject(s) - green fluorescent protein , biofilm , colocalization , gene expression , gene , microbiology and biotechnology , fluorescence , chemistry , biology , biophysics , biochemistry , bacteria , genetics , physics , quantum mechanics
Abstract The use of biofilms for the degradation of recalcitrant environmental contaminants or for the production of secondary metabolites necessitates understanding and controlling gene expression. In this work, dual labeling with green fluorescent protein (GFP) variants was used to investigate inducible gene expression in a biofilm. Colocalization of GFP emissions was used to determine regions of attached cells and to correlate structure and activity within the biofilm. The labeling strategy reported here is unique in that the two GFP signals were distinguished by differential excitation rather than differential emission.