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Rice Straw as a Support for Immobilization of Microbial Lipase
Author(s) -
de Castro Heizir F.,
de Lima Rosemar,
Roberto Inês C.
Publication year - 2001
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp010099t
Subject(s) - lipase , straw , chemistry , rice straw , microbiology and biotechnology , agronomy , triacylglycerol lipase , food science , pulp and paper industry , biology , enzyme , biochemistry , engineering
Candida rugosa lipase was covalently immobilized on rice straw activated with glutaraldehyde using poly(ethylene glycol) (PEG) as the stabilizing agent. The effects of PEG molecular weight and enzyme loading were studied according to a full 2 2 factorial design. Higher immobilization yields (>70%) were attained when the lipase loading was 95 units/mg of dry support, independent of PEG molecular weight. All derivatives showed high hydrolytic and synthetic activities. This work provides preliminary results on the use of agricultural residues as a support matrix for immobilizing lipase and on the application of the resulting derivatives to butyl butyrate synthesis as a study model.