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Cell Growth and By‐Product Formation in a Pyruvate Kinase Mutant of E. coli
Author(s) -
Zhu T.,
Phalakornkule C.,
Koepsel R. R.,
Domach M. M.,
Ataai M. M.
Publication year - 2001
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0100575
Subject(s) - mutant , pyruvate kinase , biochemistry , chemistry , wild type , escherichia coli , bacillus subtilis , biology , enzyme , glycolysis , bacteria , genetics , gene
In this paper, we report on the analysis of acid formation in an E. coli pyk mutant. The results demonstrate that acid formation is insignificant for both the wild‐type and the mutant at low glucose concentrations. However, at relatively high glucose concentrations, acid formation remains very low for the mutant but is significant for the wild‐type. This substantial reduction in acids is accompanied by an increase in CO 2 production. Moreover, unlike the B. subtilis pyk mutant, the E. coli pyk mutant did not show a substantial increase in the PEP pool.