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Lipase Immobilization into Porous Chitoxan Beads: Activities in Aqueous and Organic Media and Lipase Localization
Author(s) -
Magnin D.,
Dumitriu S.,
Magny P.,
Chornet E.
Publication year - 2001
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0100528
Subject(s) - lipase , hydrolysis , chemistry , aqueous solution , immobilized enzyme , triacylglycerol lipase , membrane , chitosan , chromatography , organic solvent , aqueous medium , chemical engineering , organic chemistry , enzyme , biochemistry , engineering
Abstract Lipases were noncovalently immobilized in Chitoxan, a polyionic hydrogel obtained by complexation between chitosan and xanthan. The properties of free and immobilized lipases have been compared. In the aqueous medium, the activity was twice as high for immobilized lipases as for free lipases. Immobilized lipases in chitoxan were able to hydrolyze triacylglycerols in three distinct organic solvent media. At the microstructural level, lipases were not distributed uniformly in the chitoxan beads. Higher concentrations of lipase were found in the outer membrane‐like layer of the beads, as compared with lower concentrations in the inner part of the beads.