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Water Content in an Engineered Dermal Replacement during Permeation of Me 2 SO Solutions Using Rapid MR Imaging
Author(s) -
Bidault Nicolas P.,
Hammer Bruce E.,
Hubel Allison
Publication year - 2001
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp010033y
Subject(s) - permeation , dimethyl sulfoxide , cryopreservation , water transport , chemistry , biophysics , bound water , aqueous solution , membrane , chemical engineering , molecule , biochemistry , organic chemistry , embryo , biology , microbiology and biotechnology , water flow , environmental engineering , engineering
The successful cryopreservation of cell and tissues typically requires the use of specialized solutions containing cryoprotective agents. At room temperature, the introduction of a cryopreservation solution can result in cell damage/death resulting from osmotic stresses and/or biochemical toxicity of the solution. For tissues, the permeation and equilibration of a cryoprotective solution throughout the tissue is important in enhancing the uniformity and consistency of the postthaw viability of the tissue. Magnetic resonance (MR) is a common nondestructive technique that can be used to quantitate the temporal and spatial composition of water and cryoprotective agents in a three‐dimensional system. We have applied a recently developed rapid NMR imaging technique to quantify the transport of water in an artificial dermal replacement upon permeation of dimethyl sulfoxide (Me 2 SO) solutions. Results indicate that the rate of water transport is slower in the presence of Me 2 SO molecules. Furthermore, the transport is concentration‐dependent, suggesting that Me 2 SO tends to retain bound water molecules in the tissue. Moreover, water transport decreases with decreasing temperature, and the presence of cells tends to increase water transport.