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Induction of Cytochrome P‐4501A1 Activity in Response to Sublethal Stresses in Microcarrier‐Attached Hep G2 Cells
Author(s) -
Mufti Naheed A.,
Shuler Michael L.
Publication year - 1995
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00036a009
Subject(s) - microcarrier , microbiology and biotechnology , chemistry , cell culture , motility , shear stress , cell , biophysics , biology , biochemistry , materials science , genetics , composite material
Cell damage for cells grown on microcarriers in suspension is a critical problem for scale‐up of microcarrier reactors. In order to study cell damage as a mechanistic process, a cellular response that is more sensitive than changes in growth and death rates and would be more closely related to cell regulatory mechanisms would be advantageous. We have observed the induction of a specific cytochrome P‐450 monooxygenase, P‐450IA1 (CYP1A1), to be a sensitive method for assessing the response of microcarrier‐attached Hep G2 cells to stress resulting from hydrodynamic shear and oxygen deprivation. The kinetics of induction and amount of CYP1Al formed in response to subtle shear stress, moderate shear, and hypoxia are described. Increased stress results in increased CYP1A1 formation.