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A Modified Growth Medium for Bacillus thuringiensis
Author(s) -
Liu Wei Ming,
Bajpai Rakesh K.
Publication year - 1995
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00035a014
Subject(s) - corn steep liquor , chemostat , bacillus thuringiensis , yeast extract , food science , salt (chemistry) , nutrient , chemistry , chemically defined medium , growth medium , fermentation , bacillales , yeast , bacteria , biology , biochemistry , bacillus subtilis , organic chemistry , in vitro , genetics
A published medium for the cultivation of Bacillus thuringiensis was modified for use in high‐density fed‐batch fermentations. This medium was diagnosed to have a complex nutrient limitation in batch and continuous cultivations. By selective additions of different medium constituents to a chemostat culture, it was established that the limitations were alleviated only by the addition of yeast extract or corn steep liquor; neither the addition of any salt nor that of individual amino acids influenced the culture behavior. In shake flask cultures, a reduction in glucose concentration and addition of corn steep liquor made the medium carbon‐limited; enrichment with corn steep liquor alone showed dual limitations. These modified media resulted in higher cell densities and showed an increase in the amounts as well as the potency of insecticidal protoxins.