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Effect of Cell Concentration on the Kinetics of Whole‐Cell Enzyme Entrapped within Calcium Alginate
Author(s) -
Lee WenChien,
Lee Ru Yue,
Ruaan Ruoh Chyu
Publication year - 1995
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00034a015
Subject(s) - calcium alginate , michaelis–menten kinetics , kinetics , chemistry , calcium , substrate (aquarium) , enzyme , cell , chromatography , population , biophysics , diffusion , kinetic energy , enzyme assay , biochemistry , thermodynamics , biology , organic chemistry , ecology , physics , demography , quantum mechanics , sociology
A method for determining the kinetics of whole‐cell enzymes entrapped within calcium alginate gel beads is presented. Diffusivities of the substrate and the product and kinetic parameters, i.e., the Michaelis constant and the maximum velocity, could be estimated by fitting experimental data to a proposed mathematical model. Immobilized cell concentration was found to be the main factor causing a decrease in maximum velocity. The influence of cell concentration in the gel beads on the variation in the maximum velocity could be isolated from the effect of cell concentration on diffusional limitation and evaluated separately. The whole‐cell enzymes became partially difficult to access by substrate when the cell population increased. The loss of enzyme accessibility resulted in a decrease in the maximum velocity and apparent initial velocity. This phenomenon of the maximum velocity varying with immobilized cell concentration has not been reported in the literature.