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Block Methacrylic Polyampholytes as Protein Displacers in Ion‐Exchange Chromatography
Author(s) -
Patrickios C. S.,
Gadam S. D.,
Cramer S. M.,
Hertler W. R.,
Hatton T. A.
Publication year - 1995
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00031a004
Subject(s) - chemistry , copolymer , ion exchange , polymer , block (permutation group theory) , chromatography , ion chromatography , ion , methacrylic acid , molecular mass , polymer chemistry , organic chemistry , enzyme , geometry , mathematics
Low molecular weight methacrylic triblock copolymers bearing a block of negatively charged (adsorbing) residues, a sequence of positively charged (repelling) units, and a middle neutral hydrophobic block were utilized successfully in anion‐exchange displacement chromatography for the resolution of a crude mixture of/β‐lactoglobulins into the A and B isoforms. Owing to their low molecular mass and ampholytic nature, these novel displacers possess the attractive process‐scale features of easy column regeneration and polymer recycling.