Ammonium‐Mediated Reduction of Plasmid Copy Number and Recombinant Gene Expression in  Escherichia coli | Zendy

Vila Pau | Zendy; Corchero José L. | Zendy; Benito Antoni | Zendy; Villaverde Antonio | Zendy

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Ammonium‐Mediated Reduction of Plasmid Copy Number and Recombinant Gene Expression in Escherichia coli

Author(s) -

Vila Pau,

Corchero José L.,

Benito Antoni,

Villaverde Antonio

Publication year - 1994

Publication title -

biotechnology progress

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.572

H-Index - 129

eISSN - 1520-6033

pISSN - 8756-7938

DOI - 10.1021/bp00030a010

Subject(s) - escherichia coli , recombinant dna , plasmid , ammonium chloride , ammonium , yield (engineering) , gene , chemistry , dna , biology , microbiology and biotechnology , biochemistry , organic chemistry , materials science , metallurgy

The effect of ammonium as a medium supplement on plasmid‐encoded recombinant β‐galactosidase synthesis was explored in Escherichia coli cells during aerobic growth in complex medium. After induction, only doses of ammonium chloride below 1 g/L are able to transiently enhance the yield. However, the presence of nontoxic ammonium chloride concentrations of up to 10 g/L results in lower values of β‐galactosidase in a concentration‐dependent fashion. A significant reduction in plasmid DNA content explains the decrease in the yield by a gene‐dosage‐involving mechanism.

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