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Ammonium‐Mediated Reduction of Plasmid Copy Number and Recombinant Gene Expression in Escherichia coli
Author(s) -
Vila Pau,
Corchero José L.,
Benito Antoni,
Villaverde Antonio
Publication year - 1994
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00030a010
Subject(s) - escherichia coli , recombinant dna , plasmid , ammonium chloride , ammonium , yield (engineering) , gene , chemistry , dna , biology , microbiology and biotechnology , biochemistry , organic chemistry , materials science , metallurgy
The effect of ammonium as a medium supplement on plasmid‐encoded recombinant β‐galactosidase synthesis was explored in Escherichia coli cells during aerobic growth in complex medium. After induction, only doses of ammonium chloride below 1 g/L are able to transiently enhance the yield. However, the presence of nontoxic ammonium chloride concentrations of up to 10 g/L results in lower values of β‐galactosidase in a concentration‐dependent fashion. A significant reduction in plasmid DNA content explains the decrease in the yield by a gene‐dosage‐involving mechanism.