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Three‐Dimensional Mammalian Cell Growth on Nonwoven Polyester Fabric Disks
Author(s) -
Petti Stacy A.,
Lages Ana C.,
Sussman Martin V.
Publication year - 1994
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00029a015
Subject(s) - polyester , nonwoven fabric , scanning electron microscope , materials science , matrix (chemical analysis) , composite material , morphology (biology) , volume (thermodynamics) , in vivo , chemistry , fiber , biology , genetics , physics , microbiology and biotechnology , quantum mechanics
Small disks of nonwoven polyester fabric (NWPF), similar to Fibra‐Cel carriers, were surface treated with strong acid and used as a high surface area support matrix for in vitro culture of anchorage dependent MRC‐5 cells. The disks can be autoclaved, and require inocula concentrations of only 2–5% of the final cell densities, which have reached 10 8 cells/mL of disk bed volume. Scanning electron microscopy photographs shown herein reveal that cells grow in a multilayered fashion between the randomly arrayed fibers of the nonwoven fabric, emulating in vivo growth.