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Modification of Lipase with Various Synthetic Polymers and Their Catalytic Activities in Organic Solvent
Author(s) -
Ito Yoshihiro,
Fujii Hajime,
Imanishi Yukio
Publication year - 1994
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00028a009
Subject(s) - lipase , catalysis , organic solvent , solvent , chemistry , organic chemistry , polymer , triacylglycerol lipase , enzyme , chemical engineering , engineering
A new enzyme modification has been devised where various vinyl monomers were graftpolymerized using enzyme‐containing aliphatic azo groups as initiators. Thus, an enzyme, lipase, which is not soluble in organic solvents, was made soluble in organic solvents. The modified enzyme catalyzed the esterification reaction in chloroform. Catalytic activity in chloroform was obtained by modification with amphiphilic polymers, such as poly( N ‐vinylpyrrolidone), as well as hydrophobic polymers, such as polystyrene and poly (methyl methacrylate). The catalytic activities of the hybrid enzymes increased linearly with increased solubility in chloroform. The hybrid enzymes were thermally stable and used repeatedly.