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Effects of Carbon Dioxide, Air Flow Rate, and Inoculation Density on the Batch Growth of Catharanthus roseus Cell Suspensions in Stirred Fermentors
Author(s) -
Van Gulik Walter M.,
Nuutila Anna Maria,
Vinke Ko L.,
Ten Hoopen Hens J. G.,
Heijnen Joseph J.
Publication year - 1994
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00027a015
Subject(s) - catharanthus roseus , aeration , growth rate , carbon dioxide , volume (thermodynamics) , inoculation , volumetric flow rate , botany , biology , bacterial growth , chemistry , chromatography , horticulture , bacteria , ecology , geometry , mathematics , physics , genetics , quantum mechanics
A high reproducibility of batch growth of Catharanthus roseus could only be achieved by using identical inoculum material. It was found that the initial growth rate of the cells increased with increasing inoculation density, whereas the maximum growth rate was not affected. Withdrawal of the conditioned medium from the inoculum resulted in a strong decline in the initial growth rate, indicating that conditioning factors affected the initial growth rate. Neither increasing the air flow rate from 0.5 to 1.5 volume of air per volume of broth‐min −1 (vvm) nor CO 2 enrichment of the aeration gas had any effect on the growth of the cells.