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Synthesis of β‐Ionone in an Aldehyde/Xanthine Oxidase/β‐Carotene System Involving Free Radical Formation
Author(s) -
Bosser Aurélie,
Belin Jean Marc
Publication year - 1994
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00026a001
Subject(s) - ionone , chemistry , xanthine oxidase , aldehyde , carotene , biochemistry , organic chemistry , food science , enzyme , catalysis
The enzymic cooxidation of β‐carotene (BC) by xanthine oxidase (XO) in aqueous solutions leads to β‐ionone (BI) and derivatives: epoxy‐β‐ionone (EPBI), dihydroactinidiolide, β‐cyclocitral, pseudoionone, etc. We demonstrate, in this article, that this is due to free radical (R⋅) formation during the first hour of stirring and the use of aldehydes as substrate. The bleaching of BC does not occur when the common substrate of XO, xanthine, is used; this proves that the superoxide anion O 2 ⋅‐ alone is not active on BC. BI formation in this case is not observed.