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Liquid‐Liquid Extraction of a Recombinant Protein with a Reverse Micelle Phase
Author(s) -
Pires M. J.,
Cabral J. M. S.
Publication year - 1993
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00024a012
Subject(s) - micelle , recombinant dna , extraction (chemistry) , chromatography , phase (matter) , chemistry , biochemistry , organic chemistry , aqueous solution , gene
Recombinant cytochrome bs was extracted to a reversed micelle phase of a cationic surfactant (hexadecyltrimethylammonium bromide, CTAB) in cyclohexane/decanol and back‐extracted to a fresh aqueous phase. Depending on the experimental conditions, i.e., temperature, pH, and ionic strength, the extraction is controlled by either hydrophobic (pH close to the p I and high ionic strength) or electrostatic (pH far from the p I and low ionic strength) interactions. The protein interacts strongly with the surfactant aggregate once it is extracted to the organic phase, and it is very difficult to back‐extract it to a new aqueous phase.