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Production of Poly(hydroxybutyrate) Homopolymer and Copolymer from Ethanol and Propanol in a Fed‐Batch Culture
Author(s) -
Alderete Jason E.,
Karl Daniel W.,
Park ChangHo
Publication year - 1993
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00023a011
Subject(s) - copolymer , valerate , polyhydroxybutyrate , chemistry , ethanol , fermentation , propionate , nuclear chemistry , starch , phosphate , polyhydroxyalkanoates , polymer chemistry , butyrate , food science , organic chemistry , polymer , bacteria , biology , genetics
Production of poly (hydroxy butyrate) (PHB) homopolymer and poly(hydroxybutyrate‐valerate) (PHBV) copolymer by a mutant strain of Alcaligenes eutrophus was studied in a 7‐L fed‐batch fermentor using ethanol and propan‐1‐o1 as sole carbon sources. Decreasing the initial phosphate concentration to 20% of the original Repaske medium allowed exhaustion of the phosphate during logarithmic growth and was an effective means of PHB(V) induction. Up to 74% PHB by weight was obtained with 63 g/L of dry cell mass. The copolymer content increased with a higher fraction of propan‐1‐o1 in the feed and reached a maximum of 35.2 mol % from pure propan‐1‐o1. Gradual oxygen limitation during the late log phase induced 21.9% PHB by weight.