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Complex Coacervate Microcapsules for Mammalian Cell Culture and Artificial Organ Development
Author(s) -
Matthew Howard W.,
Salley Steven O.,
Peterson Ward D.,
Klein Michael D.
Publication year - 1993
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00023a010
Subject(s) - coacervate , polysaccharide , polylysine , chitosan , chondroitin sulfate , carboxymethyl cellulose , cationic polymerization , chemistry , albumin , urea , biochemistry , cellulose , chromatography , glycosaminoglycan , polymer chemistry , organic chemistry , sodium
A number of combinations of anionic and cationic polymers, the majority being polysaccharides, were screened to determine their suitability for the development of alternative microcapsule formulations capable of supporting cells. The capsules were taken through a limited optimization and then evaluated on the bases of rupture strength, permeability to albumin, and ability of their components to promote the attachment, aggregation, and function of encapsulated rabbit hepatocytes. The widely used alginate—polylysine capsules were employed as a comparative standard in all tests. A number of the new formulations compared favorably with the standard, and some exhibited superior performance in specific areas. Hepatocyte function, as evaluated by the rate of urea synthesis, showed no significant differences between formulations over a 24‐h test period. One formulation, composed of the polysaccharides (carboxymethyl) cellulose, chondroitin sulfate A, chitosan, and polygalacturonate, was found to be superior to alginate—polylysine capsules in the areas investigated and supported the long‐term survival and growth of liver endothelial cells.