Effect of Silicone Antifoam on Shear Sensitivity of Hybridoma Cells in Sparged Cultures | Zendy

Van Der Pol Leo A. | Zendy; Bonarius Dick | Zendy; Van De Wouw Geert | Zendy; Tramper Johannes | Zendy

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Effect of Silicone Antifoam on Shear Sensitivity of Hybridoma Cells in Sparged Cultures

Author(s) -

Van Der Pol Leo A.,

Bonarius Dick,

Van De Wouw Geert,

Tramper Johannes

Publication year - 1993

Publication title -

biotechnology progress

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.572

H-Index - 129

eISSN - 1520-6033

pISSN - 8756-7938

DOI - 10.1021/bp00023a009

Subject(s) - defoamer , chromatography , silicone , suspension (topology) , suspension culture , shear (geology) , chemistry , bubble , cell culture , biology , materials science , dispersant , composite material , mechanics , physics , mathematics , organic chemistry , homotopy , pure mathematics , optics , genetics , dispersion (optics)

Addition of high concentrations of silicone antifoam (AF) to a suspension of hybridoma cells in a bubble column reduces the death rate when using medium without a protective component. In contrast, high AF concentrations can cause detrimental effects in a sparged medium with a protective component, such as is applied for large‐scale animal cell cultures.

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