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Effect of Silicone Antifoam on Shear Sensitivity of Hybridoma Cells in Sparged Cultures
Author(s) -
Van Der Pol Leo A.,
Bonarius Dick,
Van De Wouw Geert,
Tramper Johannes
Publication year - 1993
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00023a009
Subject(s) - defoamer , chromatography , silicone , suspension (topology) , suspension culture , shear (geology) , chemistry , bubble , cell culture , biology , materials science , dispersant , composite material , mechanics , physics , mathematics , organic chemistry , homotopy , pure mathematics , optics , genetics , dispersion (optics)
Addition of high concentrations of silicone antifoam (AF) to a suspension of hybridoma cells in a bubble column reduces the death rate when using medium without a protective component. In contrast, high AF concentrations can cause detrimental effects in a sparged medium with a protective component, such as is applied for large‐scale animal cell cultures.