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Fractional Factorial Study of Hybridoma Behavior. 2. Kinetics of Nutrient Uptake and Waste Production
Author(s) -
Gaertner John G.,
Dhurjati Prasad
Publication year - 1993
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00021a010
Subject(s) - fractional factorial design , factorial experiment , kinetics , nutrient , production (economics) , factorial , food science , chemistry , pulp and paper industry , environmental science , mathematics , econometrics , statistics , economics , organic chemistry , microeconomics , engineering , physics , mathematical analysis , quantum mechanics
A fractional factorial experimental method was used to identify important variables and variable interactions which affect nutrient uptake and waste production. The variables studied include glucose, glutamine, base medium, lactate, and ammonium. Cellular glucose uptake was stimulated by glucose and glutamine and inhibited by lactate. Glucose, base medium, and glutamine all had large stimulative effects on lactate production. Glucose uptake and lactate production both exhibited Monod‐type dependencies on the glucose concentration. Ammonium production was sensitive to the glucose and lactate concentrations at levels below about 8 and 20 mM, respectively. Mathematical descriptions of glucose uptake and of lactate and ammonium production were developed. These experimental results have significant implications for the optimization of hybridoma growth in bioreactors.