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Effect of Intraparticle Convection on the Chromatography of Biomacromolecules
Author(s) -
Frey Douglas D.,
Schweinheim Eberhard,
Horváth Csaba
Publication year - 1993
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00021a006
Subject(s) - mass transfer , convection , chromatography , chemistry , diffusion , particle (ecology) , saturation (graph theory) , packed bed , porosity , particle size , porous medium , chemical engineering , analytical chemistry (journal) , thermodynamics , organic chemistry , geology , oceanography , physics , mathematics , combinatorics , engineering
The effect of intraparticle convection in chromatographic columns packed with gigaporous particles (i. e., where d pore / d particle > 10 −2 ) on the band spreading of unretained biomacromolecules is investigated both experimentally and theoretically. A model is developed for the analysis of mass transfer in spherical particles of bidisperse pore structure when both convection and diffusion take place in the larger pores but only diffusion occurs in the smaller pores. The predictions of the model were experimentally verified. It is demonstrated that gigaporous particles have advantages over conventional porous particles (i. e., where d pore / d particle < 10 −3 ) for applications that do not require high resolving power, to bring about fast separation. This is because columns packed with gigaporous particles can be operated at high flow velocities without significant loss of efficiency due to the enhancement of mass transfer by intraparticle convection. The results of the model are used to examine the effectiveness of gigaporous column packings for rapid analytical chromatography and for the concentration and recovery of a dilute solute in a saturation‐regeneration cycle utilizing frontal chromatography.