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Characterization of Water Distribution in Cell Pellets Using Nonlabeled Sodium Thiosulfate as an Interstitial Space Marker
Author(s) -
Larroche Christian,
Besson Isabelle,
Dussap ClaudeGilles,
Bourrust Francois,
Gros JeanBernard
Publication year - 1993
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00020a016
Subject(s) - sodium thiosulfate , chemistry , filtration (mathematics) , pellets , chromatography , sodium , centrifugation , inorganic chemistry , materials science , statistics , mathematics , organic chemistry , composite material
A procedure for determination of the intracellular water content of cells using a single, nonlabeled solute as an interstitial space marker is proposed. Sodium thiosulfate, which can be accurately assayed by a tritrimetric method, is found to be a good compound for this purpose. Cells are recovered both by filtration and centrifugation; the two techniques gave the same value for internal water, i.e., 650 mg of H 2 O/g of wet matter for Corynebacterium melassecola and 390 mg of H 2 O/g of wet matter for Penicillium roquefortii spores. The methodology of data handling, based on a regression technique, is also described. It allows one to obtain very reliable results and should be useful for any marker.