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Transient Stirred‐Tank Reactors Operating with Immobilized Enzyme Systems: Analysis and Simulation Models and Their Experimental Checking
Author(s) -
Santoyo A. Bódalo,
Carrasco J. L. Gómez,
Gómez E. Gómez,
Rodriguez J. Bastida,
Morales E. Martinez
Publication year - 1993
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00020a008
Subject(s) - immobilized enzyme , reactor design , continuous stirred tank reactor , transient (computer programming) , kinetics , continuous reactor , diffusion , chemistry , enzyme kinetics , thermodynamics , michaelis–menten kinetics , biological system , enzyme , catalysis , chemical engineering , mechanics , computer science , nuclear engineering , enzyme assay , engineering , physics , organic chemistry , classical mechanics , active site , biology , operating system
A mathematical model applicable to the analysis, design, and simulation of heterogeneous enzymatic processes reacting in a stirred‐tank reactor has been formulated. The model develops the unsteady‐state design equations for such reactors and also the diffusion reaction equation in the spherical catalyst particles for reversible Michaelis—Menten kinetics. This structured general model provides an overview of almost all of the processes occurring in an immobilized enzyme reactor system. Although the analysis presented here is limited to certain situations, it can be extended to other support geometries, enzyme kinetics, and reactor configurations. To check the model, the experimental data obtained with immobilized β‐galactosidase were compared to those theoretically predicted.