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Insect Cell Hosts for Baculovirus Expression Vectors Contain Endogenous Exoglycosidase Activity
Author(s) -
Licari Peter J.,
Jarvis Donald L.,
Bailey James E.
Publication year - 1993
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00020a005
Subject(s) - exoglycosidase , spodoptera , trichoplusia , sf9 , bombycidae , glycoprotein , biology , cell culture , microbiology and biotechnology , bombyx mori , baculoviridae , oligosaccharide , endogeny , recombinant dna , biochemistry , virology , noctuidae , gene , glycan , lepidoptera genitalia , genetics , botany
Four different insect cell lines that can be used as hosts for baculovirus infection were assayed for the presence of endogenous exoglycosidases. All four cell lines, derived from Spodoptera frugiperda, Trichoplusia ni, Bombyx mori , or Malacosoma disstria , contained N ‐acetyl‐β‐glucosaminidase, N ‐acetyl‐β‐galactosaminidase, β‐galactosidase, and sialidase activities. Exoglycosidase activities were found in cell lysates as well as cell‐free supernatants from uninfected and wild‐type baculovirus infected cells. Oligosaccharide analysis of cellular glycoproteins using lectins recognizing Galβ1, 3GalNAc, Galβ1, 4GlcNAc, and NeuAcα2, 6Gal demonstrated that only Galβ1,3GalNAc was present. The demonstration that these cells contain exoglycosidases raises the possibility that the oligosaccharides of baculovirus‐expressed glycoproteins are subject to enzymatic degradation.