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Altered Distribution of Mitochondria and Actin Fibers in 3T3 Cells Cultured on Microcarriers
Author(s) -
Laney Tracey V.Du,
Cherry Robert S.,
Coppinger Justine A.,
Truskey George A.
Publication year - 1992
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00018a600
Subject(s) - microcarrier , microbiology and biotechnology , mitochondrion , 3t3 cells , actin , distribution (mathematics) , chemistry , biophysics , biology , cell , cell culture , biochemistry , genetics , transfection , mathematical analysis , mathematics
The mitochondria and actin fibers of 3T3 fibroblasts cultured on microcarriers in spinner flasks were visualized using fluorescent stains. In contrast to cells grown on planar surfaces under static or steady laminar flow conditions, cells exposed to higher levels of turbulent agitation do not form actin stress fibers. Greater agitation also leads to a more diffuse appearance of the mitochondria and a wider distribution of them throughout the cytoplasm. This response may indicate damaged mitochondria, as similar results have been reported for chemical toxins.