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Isolation of Vindoline from Catharanthus roseus by Supercritical Fluid Extraction
Author(s) -
Song KyuMin,
Park SangWoo,
Hong WonHi,
Lee Huen,
Kwak SangSoo,
Liu JangRyol
Publication year - 1992
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00018a018
Subject(s) - vindoline , catharanthus roseus , supercritical fluid , chromatography , extraction (chemistry) , supercritical fluid extraction , supercritical carbon dioxide , chemistry , high performance liquid chromatography , solvent , ethanol , organic chemistry , biology , biochemistry , genetics , chemotherapy , vinblastine
Vindoline was extracted from the leaves of Catharanthus roseus over the ranges of 35–70 °C and 100–300 bar using supercritical carbon dioxide with and without the addition of 3 wt % ethanol as a cosolvent. The vindoline contents in the extracts were determined by HPLC and identified by LC/MS. The remarkable highest vindoline concentration, 58 wt %, was obtained at the lowest temperature, 35 °C, and the highest pressure, 300 bar, of this study. The use of a cosolvent only slightly improved the extraction yields or selectivities at some experimental conditions.