Premium
Optimizing the Sample Size and the Reduced Velocity To Achieve Maximum Production Rates of Enantiomers
Author(s) -
Jacobson Stephen C.,
Felinger Attila,
Guiochon Georges
Publication year - 1992
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00018a010
Subject(s) - chromatography , enantiomer , chemistry , bovine serum albumin , production rate , recovery rate , high performance liquid chromatography , organic chemistry , industrial engineering , engineering
Optimum experimental conditions are predicted for the maximum production rate of D‐ or L‐alanine, purified to a 99% purity from a racemic mixture of their N ‐benzoyl derivatives by preparative chromatography on ionically immobilized bovine serum albumin. The experimental production rates and recovery yields measured under these conditions are in excellent agreement with the calculated values. These results validate the approach used for optimization of preparative liquid chromatography.