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Spatial Distribution of Mammalian Cells Grown on Macroporous Microcarriers with Improved Attachment Kinetics
Author(s) -
Lim HyunSoo,
Han BongKwan,
Kim JungHoe,
Peshwa Madhusudan V.,
Hu WeiShou
Publication year - 1992
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00018a003
Subject(s) - microcarrier , gelatin , chemistry , vero cell , biophysics , chemical engineering , kinetics , cell , microbiology and biotechnology , materials science , biology , biochemistry , in vitro , physics , quantum mechanics , engineering
Vero and HepG2 cells were cultivated on macroporous gelatin microcarriers prepared by the calcium carbonate inclusion method. Cell attachment to these microcarriers was slow. For HepG2 cells the subsequent growth was poor. Modification of the microcarriers by incorporation of (diethylamino)ethyl‐HCl improved HepG2 attachment and subsequent growth. Optical sectioning with confocal microscopy allowed visualization of the distribution of cells within microcarriers. In most microcarriers, cells were found to preferentially populate regions close to the external surface and some cavities in the interior. Despite the incomplete occupancy of the interior of the microcarriers, high cell concentrations were achieved.