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Application of Continuous Substrate Feeding to the ABE Fermentation: Relief of Product Inhibition Using Extraction, Perstraction, Stripping, and Pervaporation
Author(s) -
Qureshi Nasibuddin,
Maddox Ian S.,
Friedl Anton
Publication year - 1992
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00017a002
Subject(s) - fermentation , pervaporation , product inhibition , chromatography , stripping (fiber) , extraction (chemistry) , substrate (aquarium) , acetone , butanol , chemistry , lactose , volume (thermodynamics) , ethanol , permeation , pulp and paper industry , food science , membrane , biochemistry , materials science , biology , non competitive inhibition , ecology , physics , quantum mechanics , engineering , composite material , enzyme
The technique of continuous substrate feeding has been applied to the batch fermentation process using freely suspended cells, for ABE (acetone–butanol–ethanol) production. To avoid the product inhibition which normally restricts ABE production to less than 20 g/L and sugar utilization to 60 g/L, a product removal technique has been integrated into the fermentation process. The techniques investigated were liquid‐liquid extraction, perstraction, gas‐stripping, and pervaporation. By using a substrate of whey permeate, the reactor productivity has been improved over that observed in a traditional batch fermentation, while equivalent lactose utilization and ABE production values of 180 g and 69 g, respectively, have been achieved in a 1‐L culture volume.