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Scale‐Up Studies on a Defined Medium Process for Pilot Plant Production of Illicicolin by Gliocladium roseum
Author(s) -
Junker B.,
Zhang J.,
Mann Z.,
Reddy J.,
Greasham R.
Publication year - 2001
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0001718
Subject(s) - scale up , pilot plant , sucrose , environmental science , scale (ratio) , chemically defined medium , production (economics) , sterilization (economics) , liquid medium , carbon source , mixing (physics) , chemistry , food science , pulp and paper industry , process engineering , chromatography , physics , biochemistry , business , engineering , organic chemistry , finance , classical mechanics , quantum mechanics , exchange rate , economics , in vitro , foreign exchange market , macroeconomics
Illicicolin was cultivated at the 600‐L pilot scale for purposes of material generation and process development. The initial medium containing oat flour was difficult operationally as a result of excessive foaming during sterilization, so a new defined medium process (with either glucose or sucrose as the carbon source), developed at the 23‐L scale, was scaled up and improved for pilot scale needs. Pilot scale media development efforts focused on exploring the highest concentration of media (1.0× to 3.0×) that could be cultivated at the pilot scale and not be limited by mixing or oxygen mass transfer. The process was scaled up successfully and peak titers improved 7.5‐fold, from about 200 mg/L in the initial complex medium to 1500 mg/L in the final defined medium.