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Effects of FIS Overexpression on Cell Growth, rRNA Synthesis, and Ribosome Content in Escherichia coli
Author(s) -
Richins Richard,
Chen Wilfred
Publication year - 2001
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp000170f
Subject(s) - overproduction , ribosome , ribosomal rna , escherichia coli , cell growth , protein biosynthesis , biology , biochemistry , translation (biology) , chemistry , rna , microbiology and biotechnology , messenger rna , gene
The Escherichia coli DNA binding protein FIS is a transcriptional modulator involved in the regulation of many cellular processes, including the activation of rRNA synthesis. High‐level overproduction of FIS in early, mid, or late log cultures resulted in growth‐phase‐ and media‐specific variations in cell growth, rRNA synthesis, and ribosome content. FIS overproduction caused a pronounced increase in rRNA synthesis for late‐exponential cultures but a substantial reduction in cell growth and ribosome content. The addition of simple sugars such as glucose or fructose reversed these phenomena, consistent with the functional role of FIS in carbon metabolism.