Premium
Effect of Oxygen Supply on the Suspension Culture of Genetically Modified Tobacco Cells
Author(s) -
Gao Jianwei,
Lee James M.
Publication year - 1992
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00016a004
Subject(s) - erlenmeyer flask , laboratory flask , industrial fermentation , aeration , oxygen , bioreactor , suspension culture , chemistry , limiting oxygen concentration , pulp and paper industry , food science , biology , botany , chromatography , cell culture , fermentation , genetics , organic chemistry , engineering
The effect of oxygen supply on the cultivation of the genetically modified tobacco cells and the formation of a foreign protein, β‐glucuronidase (GUS), was investigated in 250‐mL Erlenmeyer flasks, a 5‐L stirred tank fermenter, and a 7‐L air‐lift fermenter. The oxygen supply was varied by using different volumes of medium in the case of the 250‐mL Erlenmeyer flask culture or by the different aeration rate in the case of the two types of fermenters tested. Higher oxygen supply stimulated cell growth and increased oxygen consumption rate, the level of phenolics, and GUS productions.