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An Improved Method for Disruption of Microbial Cells with Pressurized Carbon Dioxide
Author(s) -
Lin HoMu,
Yang Zhiying,
Chen LiFu
Publication year - 1992
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00014a012
Subject(s) - carbon dioxide , supercritical carbon dioxide , microorganism , yeast , supercritical fluid , carbon fibers , chemistry , materials science , bacteria , biology , biochemistry , organic chemistry , composite material , genetics , composite number
Disruption of microbial cells by pressurized carbon dioxide at both subcritical and supercritical temperatures has been previously investigated. This method differs in principle from other disruption techniques and was found to have potential applications for rupture of a variety of microorganisms. However, it is not as effective for some of the microbial cells, including yeast, of which the cell walls are extremely robust and rigid. This work suggests an alternative operation to improve the disruption rates of cells by repeatedly releasing the applied fluid pressure within the cells in the midst of a disruption process. The improvement is substantial at all the experimental conditions studied.