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An Amperometric Alcohol Sensor Based on Chemically Permeabilized Methylotrophic Microorganisms
Author(s) -
Chen JhyChern,
Naglak Thomas J.,
Wang Henry Y.
Publication year - 1992
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00014a011
Subject(s) - alcohol oxidase , alcohol , ethanol , yeast , methanol , pichia pastoris , chemistry , chromatography , amperometry , biochemistry , intracellular , organic chemistry , electrode , electrochemistry , gene , recombinant dna
Chemical permeabilization of the yeast Pichia pastoris NRRL Y‐11430 with 1.0% glycine and 0.1 % Triton X‐100 does not release intracellular alcohol oxidase but alters the cell envelope structure, allowing alcohol oxidase activity to be detected inside the cells (Naglak, 1990). An alcohol sensor based on the use of chemically permeabilized yeast cells was constructed. The sensor response time was within 2 min. The assay time was less than 30s by the initial rate method. The calibration curve for ethanol analysis was linear in the range 5–65 mg/L. The sensor response was almost unaffected by pH changes within the range pH 5–9. This alcohol sensor produced the highest sensitivity for methanol; the sensitivity for ethanol was about half that for methanol. The stability and other characteristics of this new alcohol sensor are also discussed in this work.