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Production of Catechol by a Bacillus stearothermophilus Transpositional Mutant
Author(s) -
Natarajan M. R.,
Oriel P.
Publication year - 1992
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00013a011
Subject(s) - catechol , mutant , electroporation , plasmid , transposable element , bacillaceae , thermophile , bacillus (shape) , bacillales , biology , geobacillus stearothermophilus , mutagenesis , microbiology and biotechnology , chemistry , biochemistry , bacteria , gene , genetics , bacillus subtilis
The phenol‐utilizing thermophile Bacillus stearothermophilus BR219 was mutagenized with transposon Tn916 introduced on a suicide plasmid using electroporation. The resulting mutant BR321 carried Tn916 integrated in the resident plasmid pGGO1 and accumulated catechol throughout the growth phase.