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Manipulation of Heterogeneous Hybridoma Cultures for Overproduction of Monoclonal Antibodies
Author(s) -
Mckinney Katherine L,
Dilwith Robert,
Belfort Georges
Publication year - 1991
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00011a010
Subject(s) - monoclonal antibody , antibody , overproduction , secretion , intracellular , microbiology and biotechnology , cell , cell culture , population , chemistry , monoclonal , biology , antigen , biochemistry , immunology , enzyme , genetics , demography , sociology
In searching for ways to manipulate heterogeneous hybridoma cell cultures (ATCC HB124) to obtain increased production of monoclonal antibodies (IgG 2a ), we have selected for a higher secreting but slower growing subpopulation using the level of fluorescent surface‐associated antibodies and a fluorescence‐activated cell sorter. Cell surface fluorescence was found to be correlated with specific antibody secretion rate over the short term but not with intracellular antibody content. Also, the specific secretion rate of a heterogeneous population of hybridoma cells grown in batch culture has been shown to be inversely correlated with an increase in either the initial cell concentration or the medium antibody concentration. Several experiments suggest that an upper limit exists for medium antibody concentration, above which antibody is degraded at the same rate at which it is produced. Should other cell lines behave similarly, strategies for overproduction of monoclonal antibodies suggested herein could be profitably used in industry.