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α‐Amylase Fermentation with Bacillus amyloliquefaciens in an Aqueous Two‐Phase System
Author(s) -
Park KyungMoon,
Wang Nam Sun
Publication year - 1991
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00011a009
Subject(s) - fermentation , bacillus amyloliquefaciens , amylase , ethylene glycol , chemistry , dextran , aqueous solution , chromatography , dilution , aqueous two phase system , phase (matter) , food science , biochemistry , enzyme , organic chemistry , physics , thermodynamics
α‐Amylase production from Bacillus amyloliquefaciens in an aqueous two‐phase system of poly(ethylene glycol) and dextran was investigated. In both batch and continuous fermentations, cells preferentially partitioned to the dextran‐rich bottom phase, glucose distributed almost evenly in both phases, and α‐amylase generally preferred the bottom phase by a small margin. A continuous fermentor with bottom‐phase recycle is proposed to achieve a significantly improved volumetric productivity of α‐amylase. Continuous α‐amylase fermentation with simultaneous product removal was demonstrated in 84 g/L poly(ethylene glycol) (MW 8000) and 84 g/L dextran (MW 71 500) at various dilution rates. Low overall cell densities economized glucose consumption. Unlike the polymer‐free fermentation, α‐amylase synthesis was not repressed even at high glucose concentrations. A properly designed continuous system with cell recycle can be considered as a nonconventional, but highly effective, method of cell immobilization.