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High‐Resolution Particle Size Analysis in Biotechnology Process Control
Author(s) -
Thomas John C.,
Middelberg Anton P. J.,
Hamel JeanFrancois,
Snoswell Mark A.
Publication year - 1991
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00010a013
Subject(s) - centrifuge , centrifugation , inclusion bodies , particle size , particle (ecology) , resolution (logic) , particle size distribution , chromatography , chemistry , inclusion (mineral) , production efficiency , materials science , biology , recombinant dna , computer science , biochemistry , physics , mineralogy , engineering , mechanical engineering , ecology , artificial intelligence , nuclear physics , gene
Many industrially important proteins can now be expressed intracellularly as insoluble protein inclusion bodies. In production, large‐scale centrifugation is commonly used to separate and recover the inclusion bodies. Recovery efficiency depends critically on the centrifuge feed rate, which must be optimized to minimize production costs. We have used a disc centrifuge photosedimentometer to make high‐resolution measurements of the particle size distribution (PSD) of the supernatant during the production of porcine somatotropin (pST) inclusion bodies. These measurements readily monitor the breakthrough of inclusion bodies into the supernatant and allow the centrifugation operation to be optimized.