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Growth and Anthocyanin Accumulation in Carrot Cell Suspension Cultures Growing on Fructose, Glucose, or Their Mixtures
Author(s) -
Zwayyed S. K.,
Frazier G. C.,
Dougall D. K.
Publication year - 1991
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00009a014
Subject(s) - anthocyanin , fructose , sugar , chemistry , carbohydrate metabolism , daucus carota , biochemistry , carbohydrate , glycolysis , food science , suspension culture , metabolism , sucrose , biology , cell culture , botany , genetics
Carrot suspension cultures have been grown on glucose, fructose, and mixtures of these two sugars and the effects of these sugars on growth and anthocyanin accumulation measured. Several parameters of growth decrease with increasing glucose in the medium. The anthocyanin accumulated remains relatively low and constant until the glucose exceeds 50% of the sugar, when the anthocyanin accumulation increases with increased glucose. The data suggest that glucose and fructose are metabolized differently in these carrot cultures despite the interconversion of their 6‐phosphates in glycolysis. Ways in which the metabolism of these sugars might differ are discussed, as is the possibility of increasing the accumulation of anthocyanin by alterations in the way these sugars are made available to the cultures.