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Biomass and Aggregation Analysis of Human Embryonic Kidney 293 Suspension Cell Cultures by Particle Size Measurement
Author(s) -
Tsao YungShyeng,
Condon Russell G. G.,
Schaefer Eugene J.,
Lindsay David A.,
Liu Zhong
Publication year - 2000
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp000088k
Subject(s) - hemocytometer , particle size , particle (ecology) , coulter counter , suspension (topology) , biological system , particle aggregation , volume (thermodynamics) , particle size distribution , embryonic stem cell , hek 293 cells , suspension culture , cell size , chemistry , cell culture , materials science , nanotechnology , biology , microbiology and biotechnology , nanoparticle , physics , mathematics , biochemistry , ecology , genetics , quantum mechanics , homotopy , pure mathematics , gene
A method has been developed to monitor the cell growth of aggregated human embryonic kidney 293 (HEK293) suspension cultures by measuring cumulative particle volume and the particle size distribution. This method employs a particle size analyzer that determines the size of individual particles by detecting their light obscuration (blockage) or scattering. Cell counts derived from the cumulative volume of the cell particle correlate well with manual cell counts from a hemacytometer at different stages of growth. This correlation was further confirmed by quantifying total cellular protein of the samples. Simultaneously, the aggregation state of the samples can also be monitored and mathematically described. Results from this study demonstrate that this simple and reproducible method allows the direct measurement of cumulative cell volume and the degree of cell aggregation, as well as an indirect assessment of cell counts.