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Production of Cyclodepsipeptides Destruxin A and B from Metarhizium anisopliae
Author(s) -
Liu BingLan,
Chen JechWei,
Tzeng YewMin
Publication year - 2000
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp000087s
Subject(s) - metarhizium anisopliae , biology , microbiology and biotechnology , psychrophile , botany , biochemistry , biological pest control , enzyme
Maltose and peptone were the best carbon and nitrogen sources for the production of destruxins from Metarhizium anisopliae . With the addition of 0.1% (w/v) β‐alanine to the basal medium, the yields of cyclodepsipeptides DA and DB were 7.2 and 279 mg/L, respectively, which was 2‐fold higher than that of control experiment. Response surface methodology (RSM) was applied to optimize the compositions of maltose, peptone, β‐alanine, and glucose used in a shaker‐flask cultivation of M . anisopliae for the production of DA and DB. Estimated optimal compositions for the DA production were maltose 2.58%, peptone 0.72%, β‐alanine 0.02%, and glucose 0.55%. The predicted DA yield was 18.5 mg/L. On the other hand, the optimal compositions for DB production were maltose 2.51%, peptone 0.75%, β‐alanine 0.02%, and glucose 0.43%. A maximum DB yield of 232 mg/L was predicted. These were confirmed by cultivation experiments conducted at the optimized conditions for maximum destruxins production in a shaker‐flask. Furthermore, a modest high level of DA (49 mg/L) and DB (268 mg/L) yields were obtained by employing the response surface methodology optimized DB production medium in a no‐baffle, stirred‐tank fermentor.