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Using the Microcyte Flow Cytometer To Monitor Cell Number, Viability, and Apoptosis in Mammalian Cell Culture
Author(s) -
Harding Claire L.,
Lloyd David R.,
McFarlane Caroline M.,
AlRubeai Mohamed
Publication year - 2000
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp0000813
Subject(s) - biotinylation , flow cytometry , apoptosis , viability assay , allophycocyanin , cell culture , cell , biology , camptothecin , population , microbiology and biotechnology , annexin , cell counting , electroporation , cell cycle , biochemistry , genetics , phycocyanin , medicine , bacteria , cyanobacteria , environmental health , gene
Abstract The Microcyte is a novel, portable flow cytometer based on diode laser technology whose use has been established for yeast and bacterial analysis. We present data that demonstrate its suitability for routine mammalian cell counting and viability determination. To extend its range of applications in the field of animal cell culture biotechnology, a test to determine the number of apoptotic cells present has been developed for use with the instrument. Apoptosis was induced in hybridoma cell cultures by treatment with camptothecin. Apoptotic cells were labeled with biotinylated Annexin V and then visualized using a streptavidin‐allophycocyanin conjugate. Their numbers were counted, and the cell size of the apoptotic cell population was determined using the Microcyte.