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Protein Recovery from Reversed Micellar Solutions through Contact with a Pressurized Gas Phase
Author(s) -
Phillips John B.,
Nguyen Huyen,
John Vijay T.
Publication year - 1991
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00007a007
Subject(s) - clathrate hydrate , dissolution , chemistry , ionic strength , solvent , phase (matter) , chemical engineering , hydrate , ionic bonding , chromatography , aqueous solution , organic chemistry , ion , engineering
We describe a new process for the recovery of encapsulated protein from reversed micellar solution in concentrated form. The method involves desolubilization of the protein by decreasing solvent density through gas dissolution. Under appropriate thermodynamic conditions, the micellar water pool can be converted to clathrate hydrates. Protein recovery is facilitated by clathrate hydrate formation, which causes the desolubilized protein to exist in a solid phase, distinct from the micellar supernatant. The process is carried out without any ionic strength or pH modification.