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Overloaded Hollow‐Fiber Liquid Chromatography
Author(s) -
Ding Hongbing,
Cussler E. L.
Publication year - 1990
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1021/bp00006a010
Subject(s) - chromatography , dimensionless quantity , chemistry , solvent , stationary phase , fiber , phase (matter) , drop (telecommunication) , dispersion (optics) , diffusion , adsorption , pressure drop , thermodynamics , organic chemistry , telecommunications , physics , computer science , optics
Liquid chromatography in hollow fibers can separate solutes like flavors and proteins by using a stationary phase of organic solvent, sometimes containing reversed micelles. Such separations, which have a much smaller pressure drop than equivalent separations in packed beds, show dispersion consistent with chromatographic theories at low flows and dilute feeds. These separations behave less predictably at high flows and concentrated feeds, which overload the hollow fibers. The results for flavors correlate well with the Graetz number, consistent with available theories of chromatography and adsorption. The results for proteins correlate poorly with the Graetz number but better with a dimensionless flux based on facilitated diffusion in the stationary phase.